Codon-Optimized Bacterial Genes Improve l-Arabinose Fermentation in Recombinant Saccharomyces cerevisiae
نویسندگان
چکیده
منابع مشابه
Codon-optimized bacterial genes improve L-Arabinose fermentation in recombinant Saccharomyces cerevisiae.
Bioethanol produced by microbial fermentations of plant biomass hydrolysates consisting of hexose and pentose mixtures is an excellent alternative to fossil transportation fuels. However, the yeast Saccharomyces cerevisiae, commonly used in bioethanol production, can utilize pentose sugars like l-arabinose or d-xylose only after heterologous expression of corresponding metabolic pathways from o...
متن کاملEffect of arabinose on glucose fermentation by Saccharomyces cerevisiae.
It has recently been demonstrated (Borzani et al., 1956) that the mash obtained from Araucaria brasiliensis seeds by acid hydrolysis, when fermented by Saccharomyces cerevisiae, give alcohol yields practically constant at pH values from 2.9 to 7.2. This fact represents a considerable increase of the yeast optimum pH interval. It has also been observed (Borzani et al., 1956) that glucose and ara...
متن کاملArabinose and xylose fermentation by recombinant Saccharomyces cerevisiae expressing a fungal pentose utilization pathway
BACKGROUND Sustainable and economically viable manufacturing of bioethanol from lignocellulose raw material is dependent on the availability of a robust ethanol producing microorganism, able to ferment all sugars present in the feedstock, including the pentose sugars L-arabinose and D-xylose. Saccharomyces cerevisiae is a robust ethanol producer, but needs to be engineered to achieve pentose su...
متن کاملImprovement of L-Arabinose Fermentation by Modifying the Metabolic Pathway and Transport in Saccharomyces cerevisiae
The L-arabinose utilization pathway was established in Saccharomyces cerevisiae, by expressing the codon-optimized araA, araB, and araD genes of Lactobacillus plantarum. After overexpressing the TAL1, TKL1, RPE1, RKI1, and GAL2 genes and adaptive evolution, the L-arabinose utilization of the recombinant strain became efficient. The resulting strain displayed a maximum specific growth rate of 0....
متن کاملEngineering of Saccharomyces cerevisiae for efficient anaerobic alcoholic fermentation of L-arabinose.
For cost-effective and efficient ethanol production from lignocellulosic fractions of plant biomass, the conversion of not only major constituents, such as glucose and xylose, but also less predominant sugars, such as l-arabinose, is required. Wild-type strains of Saccharomyces cerevisiae, the organism used in industrial ethanol production, cannot ferment xylose and arabinose. Although metaboli...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2008
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.02395-07